Exicycler™ 96 Real-Time Quantitative Thermal Block from Bioneer, Inc.

Product Specs
ItemExicycler™ 96 Real-Time Quantitative Thermal Block
CompanyBioneer, Inc.
Price Get Pricing Get Quote for this Product
Catalog NumberA-2060-1
Dimensions(WxDxH) 14.0 x 18.5 x 21.3 in
QuantityEA
ApplicationsReal-time PCR, Gene Detection, Quantification of Gene Expression, miRNA Expression Analysis, Viral Load Analysis, Pathogen Detection, Genotype Analysis, SNP Detection, Genetic Disease Detection, DNA Methylation Study, 5-color Multiplexing
Volume20 to 100 µl
Wells96 wells
Lid TypeHeated
Temperature Range4.0 °C to 99.9 °C
Temperature Uniformity± 0.3 °C (or ± 0.54 °F)
Weight86.0 lbs
Detection16-bit 2D CCD, 5 filter sets
Heating Rate5 °C/sec
Channels5

Features

Temperature gradient

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Bioneer, Inc.

155 Filbert St.

Unit 216

Oakland, CA 94607

United States

Phone: (877) 264 - 4300
Fax: (510) 865 - 0350

Email: order.usa@bioneer.us.com

Company Profile

Website: http://www.bioneer.com/

(1) Synergistic Antioxidant and Anti-Inflammatory Activities of Kale Juice Fermented with Limosilactobacills reuteri EFEL6901 or Limosilactobacills fermentum EFEL6800Antioxidants (Basel)October 12, 2023Ga Yun Kim, Seul-Ah Kim, Sun Young Kong, Hyunbin Seong, Jae-Han Bae, Nam Soo Hanof the reverse transcription process across different samples. Real-time PCR was conducted using the Exicycler 96 Real-Time Quantitative Thermal Block (Exicycler 96; Bioneer, Daejeon, Korea). The mixtures containing synthesized cDNA, 10 pmol of specific

(2) The effect of growth hormone on ovarian function recovery in a mouse model of ovarian insufficiencyFront Endocrinol (Lausanne)October 3, 2023Su Mi Kim, Jung Young Yoo, Yeon Hee Hong, Jaewang Lee, Ji Hyang Kim, Jung Ryeol Leemix. All qRT-PCR amplifications and analyses were conducted using an Accupower qPCR PreMix kit in an Exicycler 96 Real-Time Quantitative Thermal Block (Bioneer). The PCR conditions were as follows: initial denaturation of the template DNA at 95°C for

(3) Characteristics of the Salivary Microbiota in Periodontal Diseases and Potential Roles of Individual Bacterial Species To Predict the Severity of Periodontal DiseaseMicrobiology SpectrumJune 15, 2023Suk Ji, Joong-Ki Kook, Soon-Nang Park, Yun Kyong Lim, Geum Hee Choi, Jae-Suk Jungdeduced from previous studies (40–42). qPCR was performed with AccuPower GreenStar qPCR PreMix using an Exicycler 96 real-time quantitative thermal block (Bioneer). Each PCR was performed with a total volume of 50 μL containing 3 μL each of the forward and

(4) Development of a rapid and sensitive real-time diagnostic assay to detect and quantify Aphanomyces invadans, the causative agent of epizootic ulcerative syndromePLOS ONEJune 15, 2023Diem Tho Ho, Nameun Kim, Yoonhang Lee, Dongbin Yun, MinJi Sung, El-Matbouli Mansour, P. K. Pradhan, Neeraj Sood, Wi-Sik Kim, Chan-Il Park, et al.dilution was analyzed in duplicate, with two runs per day over five consecutive days using three different Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer, Korea) instruments (EXI-05C-0601022, EXI-05C-0601016, and EXI-05M-0802030), and three different

(5) Comprehensive Analysis of Rice Seedling Transcriptome during Dehydration and RehydrationInternational Journal of Molecular SciencesMay 8, 2023So Young Park, Dong-Hoon JeongJapan). qRT-PCR was performed using a KOD SYBR qPCR Mix kit (Toyobo, QKD-201; Osaka, Japan) with an Exicycler 96 Real-Time Quantitative Thermal Block System (Bioneer, Daejeon, Republic of Korea). The expression levels were calculated from two technical

(6) LAPTM5 regulated by FOXP3 promotes the malignant phenotypes of breast cancer through activating the Wnt/β‑catenin pathwayOncology reportsMarch 1, 2023Han S, Jin X, Hu T, Chi F.and 2X Power Taq PCR Master Mix (BioTeke Corporation), and the mixture was subjected to RT-qPCR in an Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Corporation) according to the manufacturer's instructions. The mRNA expression levels of the

(7) Distribution Analysis of Candida albicans according to Sex and Age in Clinical Specimen Testing for Sexually Transmitted DiseasesJournal of Microbiology and BiotechnologyJanuary 28, 2023Jae Eun Choi, Jae-Sik Jeon, Jae Kyung KimAnalysis Real-time PCR analysis was performed using the AccuPower STI4C-Plex Real-Time PCR Kit with an Exicycler 96 Real-Time Quantitative Thermal Block (Bioneer), according to the manufacturer’s instructions. The PCR mixture was prepared by adding 1 μl

(8) Development and Characterization of Synthetic Norovirus RNA for Use in Molecular Detection MethodsAnnals of Laboratory MedicineJanuary 1, 2023Eun-Jung Cho, Younggil Cha, Su Kyung Lee, Han-Sung Kim, Jae-Seok Kim, Eun Jin Lee, Nuri Lee, Ki Ho Hong, Hee Jin Huh, Young Joo Cha, et al.RT-PCR Kit for CFX96 (Bioneer) on the CFX96 system, and AccuPower Norovirus Real-Time RT-PCR Kit on an Exicycler 96 Real-Time Quantitative Thermal Block (Bioneer). Standard curves were plotted using five concentrations of standard materials to quantify

(9) High frequency of KRAS and EGFR mutation profiles in BRAF-negative thyroid carcinomas in IndonesiaBMC Research NotesDecember 12, 2022Didik Setyo Heriyanto, Vincent Laiman, Nikko Vanda Limantara, Widyan Putra Anantawikrama, Fara Silvia Yuliani, Rita Cempaka, Sumadi Lukman AnwarDetection Kit (Amoy Diagnostics, Xiamen, China) [15]. Quantitative PCR was performed using Bioneer Exicycler™ 96 Real-Time Quantitative Thermal Block. The assays and PCR condition were as manufacturer’s recommendation. Briefly, region of interest within

(10) Coinfections with multiple sexually transmitted pathogens in Republic of Korea, 2018–2020Journal of Clinical Laboratory AnalysisOctober 1, 2022Sun Jung Lee, Tae Su Jang, Jae‐Sik Jeon, Jae Kyung KimAccuPower STI8B‐Plex Real‐Time PCR kit (Exicycler 96, Bioneer, Daejeon, Republic of Korea) with an Exicycler 96 Real‐Time Quantitative Thermal Block (Exicycler 96, Bioneer, Daejeon, Republic of Korea) according to the manufacturer's protocol. The amplification

(11) Harpagoside attenuates local bone Erosion and systemic osteoporosis in collagen-induced arthritis in miceBMC Complementary Medicine and TherapiesAugust 10, 2022Ju-Young Kim, Yoon-Hee Cheon, Sung-Jun Ahn, Sung Chul Kwak, Chong Hyuk Chung, Chang Hoon Lee, Myeung Su LeeCo., Daejeon, Korea), 10 pmol of forward primer, 10 pmol of reverse primer, and 1 μg of cDNA using an Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer Co.) as described previously [24]. The follwing mouse-specific primers used: mouse TRAP, forward

(12) The Comparative Analysis of Two RT-qPCR Kits for Detecting SARS-CoV-2 Reveals a Higher Risk of False-Negative Diagnosis in Samples with High Quantification Cycles for Viral and Internal GenesCanadian Journal of Infectious Diseases and Medical MicrobiologyJuly 5, 2022Roberto Luraschi et al.reaction. The Bioneer Exicycler™ 96 Realtime Quantitative Thermal Block (Bioneer Co., Reference code: A-2060-1) was used for this assay. Data were extracted using the Bioneer Exicycler™ 96 software. 2.7. Ethics

(13) Comparative Performance Evaluation of FilmArray BioFire RP2.1 and MAScIR 2.0 Assays for SARS-CoV-2 DetectionAdvances in VirologyJune 1, 2022Sophia Tazi, Hakima Kabbaj, Jalila Zirar, Amal Zouaki, Ghizlane El Amin, Othman El Himeur, Myriam Seffar7500 FAST Real-Time PCR System (Applied Biosystems™), QuantStudio™ 5 DX Real-Time PCR System Applied Biosystems™, and Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer™), according to the following protocol: 5 minutes at 50°C for reverse transcription, 20 seconds

(14) Age and sex trends of Gardnerella vaginalis infection in patients with sexually transmitted infections in KoreaIranian Journal of MicrobiologyFebruary 28, 2022Young Sam Yuk, Jae Eun Choi, Jae Kyung Kimanalysis. Real-time PCR analysis was performed using the AccuPower STI4C-Plex Real-Time PCR Kit with an Exicycler 96 Real-Time Quantitative Thermal Block (Bioneer) according to the manufacturer’s protocol. The amplification protocol consisted of one cycle

(15) Accuracy of Real-Time Polymerase Chain Reaction in COVID-19 PatientsMicrobiology SpectrumFebruary 23, 2022Merlin Jayalal Lawrence Panchali, Hyeon Jeong Oh, You Mi Lee, Choon-Mee Kim, Misbah Tariq, Jun-Won Seo, Da Young Kim, Na Ra Yun, Dong-Min Kimcarried out according to the manufacturer’s specifications. For iNP target, RT-qPCR was performed in Exicycler 96 Real-Time Quantitative Thermal Block (Bioneer, Daejeon, Korea), and for Kogene kits CFX96 Touch Real-Time PCR Detection System (CA, USA)

(16) Laboratory investigations of herpes simplex virus-1 and -2 clinical samples in KoreaOsong Public Health and Research PerspectivesDecember 31, 2021Eun Ju Oh, Young Sam Yuk, Jae Kyung KimAnalysis Real-time PCR analysis was performed using the AccuPower STI8B-Plex Real-Time PCR kit with an Exicycler 96 Real-Time Quantitative Thermal Block (Exicycler 96; Bioneer), according to the manufacturer’s protocol. The amplification protocol comprised

(17) Mycoplasma genitalium and Mycoplasma hominis infection in south Korea during 2018-2020Iranian Journal of MicrobiologyDecember 13, 2021Eun Ju Oh, Tae Su Jang, Jae Kyung KimDaejeon, South Korea) and AccuPower® STI8B-Plex Real-Time PCR Kit (Bioneer, Daejeon, South Korea) on an Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer, Daejeon, South Korea) according to the manufacturer’s protocol. Undiluted DNA samples (>20

(18) Potential Efficacy of Chitosan-Poly (Lactide-Co-Glycolide)-Encapsulated Trivalent Immersion Vaccine in Olive Flounder (Paralichthys olivaceus) Against Viral Hemorrhagic Septicemia Virus, Streptococcus parauberis Serotype I, and Miamiensis avidus (Scuticociliate)Frontiers in ImmunologyDecember 2, 2021Sajal Kole, Showkat Ahmad Dar, Su-Mi Shin, Hyeon-Jong Jeong, Sung-Ju Jungare listed in Supplementary File 1 . To analyze gene expression, real-time PCR was performed in an Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer, Korea) using SYBR Green AccuPower® PCR PreMix (Bioneer). Relative quantification of immune

(19) Anti-Osteoporosis Effects of the Eleutherococcus senticosus, Achyranthes japonica, and Atractylodes japonica Mixed Extract Fermented with NurukNutrientsOctober 30, 2021So Young Eun, Yoon-Hee Cheon, Gyeong Do Park, Chong Hyuk Chung, Chang Hoon Lee, Ju-Young Kim, Myeung Su Leemanufacturer’s instructions (Invitrogen, Carlsbad, CA, USA). Quantitative RT-PCR was carried out on the Exicycler 96 Real-Time Quantitative Thermal Block (Bioneer Co., Daejeon, Korea) using the SYBR®® Green Premix (Bioneer Co., Daejeon, Korea) with specific

(20) HE4, A New Potential Tumor Marker for Early Diagnosis and Predicting of Breast Cancer ProgressionIranian journal of pathologyJune 1, 2021Mirmohseni Namini N, Abdollahi A, Movahedi M, Emami Razavi A, Saghiri R.µL forward primer (10 pmol), and 0.5 µL reverse primer (10 pmol). The amplification was performed by Exicycler™ 96 Real-Time Quantitative Thermal Block (Bioneer, Daedeok-gu, Daejeon, Republic of Korea) under following conditions: incubation at 95˚C for

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